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jetprime dna/sirna  (Sartorius AG)


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    Structured Review

    Sartorius AG jetprime dna/sirna
    Jetprime Dna/Sirna, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 11113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jetprime/jetPRIME+DNA%2FsiRNA/custom%40101000027%4042639794
    Average 99 stars, based on 11113 article reviews
    jetprime dna/sirna - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Method for producing t cells modified by chimeric antigen receptor
    Article Snippet: .. Specific steps are as follows. (1) The 293FT cell culture flask (T175) that had reached 80%-90% was taken out from the cell incubator in 5% CO2 at 37° C., the cells were collected and washed after digestion, 4.5×106 cells and 9 mL of DMEM complete medium (purchased from Gibco, product catalog No.: 11965-084) were added to each 10 cm-cell culture dish and gently shaken, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. (2) On the second day of culturing, the following reagents were added to each culture dish: 500 μL of jetPRIME® buffer (purchased from Polyplus Transfection Co., product catalog No.: B161116), 6 μg of recombinant lentivirus plasmid, 3 μg of psPAX2 (purchased from Wuhan Miaoling Biotechnology Co., Ltd., product catalog No.: P026) and 1.5 μg of pMD2.G (purchased from Guangzhou Geneseed Biotechnology Co., Ltd., product catalog No.: 161220L08) and then mixed well, and then jetPRIME® (purchased from Polyplus Transfection Co., product catalog No.: 114-15) was added to the system at 25 μL per 10 cm-culture dish and mixed well, and the resulting mixture was left to stand at room temperature for 10 min to obtain a mixed solution. (3) The 293FT cells used for packaging viruses were taken out of the cell incubator in 5% CO2 at 37° C., the mixed solution was added to each culture dish and the shaken gently, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. for continuous culturing. ..

    Recombinant:

    Article Title: Method for producing t cells modified by chimeric antigen receptor
    Article Snippet: .. Specific steps are as follows. (1) The 293FT cell culture flask (T175) that had reached 80%-90% was taken out from the cell incubator in 5% CO2 at 37° C., the cells were collected and washed after digestion, 4.5×106 cells and 9 mL of DMEM complete medium (purchased from Gibco, product catalog No.: 11965-084) were added to each 10 cm-cell culture dish and gently shaken, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. (2) On the second day of culturing, the following reagents were added to each culture dish: 500 μL of jetPRIME® buffer (purchased from Polyplus Transfection Co., product catalog No.: B161116), 6 μg of recombinant lentivirus plasmid, 3 μg of psPAX2 (purchased from Wuhan Miaoling Biotechnology Co., Ltd., product catalog No.: P026) and 1.5 μg of pMD2.G (purchased from Guangzhou Geneseed Biotechnology Co., Ltd., product catalog No.: 161220L08) and then mixed well, and then jetPRIME® (purchased from Polyplus Transfection Co., product catalog No.: 114-15) was added to the system at 25 μL per 10 cm-culture dish and mixed well, and the resulting mixture was left to stand at room temperature for 10 min to obtain a mixed solution. (3) The 293FT cells used for packaging viruses were taken out of the cell incubator in 5% CO2 at 37° C., the mixed solution was added to each culture dish and the shaken gently, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. for continuous culturing. ..

    Plasmid Preparation:

    Article Title: Method for producing t cells modified by chimeric antigen receptor
    Article Snippet: .. Specific steps are as follows. (1) The 293FT cell culture flask (T175) that had reached 80%-90% was taken out from the cell incubator in 5% CO2 at 37° C., the cells were collected and washed after digestion, 4.5×106 cells and 9 mL of DMEM complete medium (purchased from Gibco, product catalog No.: 11965-084) were added to each 10 cm-cell culture dish and gently shaken, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. (2) On the second day of culturing, the following reagents were added to each culture dish: 500 μL of jetPRIME® buffer (purchased from Polyplus Transfection Co., product catalog No.: B161116), 6 μg of recombinant lentivirus plasmid, 3 μg of psPAX2 (purchased from Wuhan Miaoling Biotechnology Co., Ltd., product catalog No.: P026) and 1.5 μg of pMD2.G (purchased from Guangzhou Geneseed Biotechnology Co., Ltd., product catalog No.: 161220L08) and then mixed well, and then jetPRIME® (purchased from Polyplus Transfection Co., product catalog No.: 114-15) was added to the system at 25 μL per 10 cm-culture dish and mixed well, and the resulting mixture was left to stand at room temperature for 10 min to obtain a mixed solution. (3) The 293FT cells used for packaging viruses were taken out of the cell incubator in 5% CO2 at 37° C., the mixed solution was added to each culture dish and the shaken gently, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. for continuous culturing. ..

    Article Title: A novel adenovirus 19K/IX protein promotes infection by preventing proteasomal degradation of tyrosine-ubiquitinated capsid protein pIX
    Article Snippet: Plasmid encoding HA-Ubiquitin was purchased from Addgene (#17608), the His-Ubiquitin expressing plasmid has been described before [ ]. .. All plasmid transfections were performed with the jetPRIME (Polyplus) transfection reagent according to the manufacturer’s protocol. .. All plasmid transfections were performed with the jetPRIME (Polyplus) transfection reagent according to the manufacturer’s protocol.

    Article Title: Targeting UBE2T by β-elemene inhibits prostate cancer stem cells and bone metastasis by blocking the TRIM28/pGSK3β/β-catenin signaling.
    Article Snippet: .. Briefly, Plasmid transfection was performed using jetPRIME (Polyplus, Strasbourg, France). siRNA interference assay siRNA interference assay was performed to knockdown UBE2T expression using LipofectamineTM 3000 reagent (Invitrogen). ..

    Transfection:

    Article Title: A novel adenovirus 19K/IX protein promotes infection by preventing proteasomal degradation of tyrosine-ubiquitinated capsid protein pIX
    Article Snippet: Plasmid encoding HA-Ubiquitin was purchased from Addgene (#17608), the His-Ubiquitin expressing plasmid has been described before [ ]. .. All plasmid transfections were performed with the jetPRIME (Polyplus) transfection reagent according to the manufacturer’s protocol. .. All plasmid transfections were performed with the jetPRIME (Polyplus) transfection reagent according to the manufacturer’s protocol.

    Article Title: FOXC2 represses NFAT1-dependent transcription through a DNA-facilitated protein–protein interaction
    Article Snippet: .. The cells were transfected with pcDNA3.1–NFAT1 and pcDNA3.1–FOXC2 plasmids for 48 h using jetPRIME (Polyplus, 101000046) according to the manufacturer’s instructions. .. The total protein of HEK 293T cells was lysed by 1% Triton X-100 (Sigma–Aldrich) buffer supplemented with protease inhibitor cocktail (Roche, 11697498001).

    Article Title: Targeting UBE2T by β-elemene inhibits prostate cancer stem cells and bone metastasis by blocking the TRIM28/pGSK3β/β-catenin signaling.
    Article Snippet: .. Briefly, Plasmid transfection was performed using jetPRIME (Polyplus, Strasbourg, France). siRNA interference assay siRNA interference assay was performed to knockdown UBE2T expression using LipofectamineTM 3000 reagent (Invitrogen). ..

    Article Title: The proximal N-terminus of IRAG is required for potentiation of HCN4 channels
    Article Snippet: .. Transient transfection of HCN4 constructs and/or IRAG was performed using Fugene6 (Promega, Madison, WI) or JetPrime (Polyplus-Sartorius, Illkirch, France), both using manufacturer’s instructions. ..

    Article Title: SNHG7 interacts with PCBP2 to promote CDKN2A expression and modulate cuproptosis in colorectal cancer
    Article Snippet: .. They were transfected into SW480 and RKO cells according to the manufacturer's instructions by jetPRIME (Polyplus-transfection, France). ..

    Article Title: DNA-PK interacts with cyclic dinucleotides and inhibits type I interferon responses
    Article Snippet: Flow cytometry data were then analyzed using the NovoExpress software (Agilent), while fluorescence microscopy data were analyzed with Harmony software (Revvity). .. T98G cells grown on coverslips were transfected with 1 μg/ml 2′3′-cGAMP–iFluor 488 (#20320; AAT BioQuest) or DMSO (control) using JetPRIME (Sartorius). .. After 6 h, cells were washed with PBS, fixed with 4% formaldehyde for 20 min, then blocked/permeabilized in PBS supplemented with 0.5% Triton X-100 and 5% BSA for 1 h. Cells were incubated with anti–DNA-PK (#ab32566; Abcam, 1:100) for 1 h, washed, and exposed to Alexa Fluor 594 goat anti-rabbit (# R37117 ; Invitrogen) for 1 h. Nuclei were stained with DAPI (1 μg/ml, 10 min) and then mounted with VECTASHIELD PLUS (#H-1900; Vector).

    Article Title: FOXC2 represses NFAT1-dependent transcription through a DNA-facilitated protein-protein interaction.
    Article Snippet: .. The cells were transfected with pcDNA3.1 –NFAT1 and pcDNA3.1 –FOXC2 plasmids for 48 h using jetPRIME (Polyplus, 101000046) according to the manufacturer’s instructions. .. The total protein of HEK 293T cells was lysed by 1% Triton X-100 (Sigma –Aldrich) buffer supplemented with protease inhibitor cocktail (Roche, 11697498001).

    Knockdown:

    Article Title: Targeting UBE2T by β-elemene inhibits prostate cancer stem cells and bone metastasis by blocking the TRIM28/pGSK3β/β-catenin signaling.
    Article Snippet: .. Briefly, Plasmid transfection was performed using jetPRIME (Polyplus, Strasbourg, France). siRNA interference assay siRNA interference assay was performed to knockdown UBE2T expression using LipofectamineTM 3000 reagent (Invitrogen). ..

    Expressing:

    Article Title: Targeting UBE2T by β-elemene inhibits prostate cancer stem cells and bone metastasis by blocking the TRIM28/pGSK3β/β-catenin signaling.
    Article Snippet: .. Briefly, Plasmid transfection was performed using jetPRIME (Polyplus, Strasbourg, France). siRNA interference assay siRNA interference assay was performed to knockdown UBE2T expression using LipofectamineTM 3000 reagent (Invitrogen). ..

    Construct:

    Article Title: The proximal N-terminus of IRAG is required for potentiation of HCN4 channels
    Article Snippet: .. Transient transfection of HCN4 constructs and/or IRAG was performed using Fugene6 (Promega, Madison, WI) or JetPrime (Polyplus-Sartorius, Illkirch, France), both using manufacturer’s instructions. ..

    Control:

    Article Title: DNA-PK interacts with cyclic dinucleotides and inhibits type I interferon responses
    Article Snippet: Flow cytometry data were then analyzed using the NovoExpress software (Agilent), while fluorescence microscopy data were analyzed with Harmony software (Revvity). .. T98G cells grown on coverslips were transfected with 1 μg/ml 2′3′-cGAMP–iFluor 488 (#20320; AAT BioQuest) or DMSO (control) using JetPRIME (Sartorius). .. After 6 h, cells were washed with PBS, fixed with 4% formaldehyde for 20 min, then blocked/permeabilized in PBS supplemented with 0.5% Triton X-100 and 5% BSA for 1 h. Cells were incubated with anti–DNA-PK (#ab32566; Abcam, 1:100) for 1 h, washed, and exposed to Alexa Fluor 594 goat anti-rabbit (# R37117 ; Invitrogen) for 1 h. Nuclei were stained with DAPI (1 μg/ml, 10 min) and then mounted with VECTASHIELD PLUS (#H-1900; Vector).



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    Sartorius AG jetprime dna/sirna
    Jetprime Dna/Sirna, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jetprime/jetPRIME+DNA%2FsiRNA/custom%40101000027%4042639794
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